National Refresher Course in Plant Biotechnology

Sponsored by the Andhra Pradesh Netherlands Biotechnology Programme

LABORATORY  MANUAL FOR THE  TRAINING COURSE

•  Production of transgenic plants by Agrobacterium mediated gene transfer
•  Particle gun mediated Genetic transformation:
•  Protoplast isolation, culture and fusion
•  Plant Genomic DNA Isolation
•  Southern Blotting
•  RNA isolation from Plant Tissue
•  Polymerase Chain Reaction
•  RT-PCR
•  Restriction digestion of vector, insert elution, ligation and transformation
•  Plasmid Isolation
•  Analysis of recombinant clones